Nikon introduces the CFI Plan Apochromat Lambda D series objectives for biological microscopes

Supports large field-of-view observation and corrects chromatic aberration over a wide wavelength range

December 23, 2021

TOKYO - Nikon Corporation (Nikon) is pleased to announce the release of 7 models of CFI Plan Apochromat Lambda D series objectives for biological microscopes, that support observation with a large 25 mm field-of-view^*1 and enable chromatic aberration correction across a wide wavelength range.

• *1The size of the image that can be obtained from the microscope.

Release Overview

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Development Background

In the field of life science research, there is high demand for improving observation efficiency by capturing the specimen clearly with a large field of view and observing fine structures. To meet these needs, Nikon has improved the current CFI Plan Apochromat Lambda series objectives for biological microscopes that support clear imaging over a large field of view, resulting in the new CFI Plan Apochromat Lambda D series. This series supports observation with a large field-of-view of 25 mm, corrects chromatic aberration over a wider wavelength range than the conventional model, and achieves high numerical aperture^*2 and high resolution.
Higher resolution images up to the periphery of the field of view can be acquired when combined with the “Digital Sight 10” microscope camera that will be released concurrently.

• *2A numerical value that indicates the solid angle with which an objective can collect light. The larger the number, the wider the solid angle of light that can be captured, resulting in brighter and higher resolution images.

Main Features

1. Imaging a large field of view, bright and clear, and improving observation efficiency

By adopting the newly developed high refractive index glass, it is possible to suppress the decrease in the amount of light at the periphery of the field of view and capture the observation target brightly and clearly all the way to the edge of the field of view. High visibility and efficient observation with a 25 mm large field of view, and high throughput, can be achieved.

Sliced specimen of mouse kidney
Images acquired using a conventional objective (left) and a CFI Plan Apochromat Lambda D 10X objective (right)

2. Providing objectives for a wide range of wavelengths

This series of lenses adopts anomalous dispersion glass and corrects chromatic aberration that causes color bleeding in a wavelength range from the visible to the near infrared (405 nm to 850 nm). Since the Lambda D series can support reagents with a wide range of wavelengths with a single objective, it is no longer necessary to use a different objective for each wavelength.

3. Achieves high resolution with high numerical aperture

High numerical aperture has been achieved by processing the lens edge to be thinner and with higher precision, and enlarging the effective diameter of the lens. It enables both a high numerical aperture and a large field of view, as well as bright and high-resolution observations, clearly capturing the fine structure of the specimen.

Main Specifications

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The information is current as of the date of publication. It is subject to change without notice.

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