Imaging Software


  1. Overview
  2. Key Features
  3. Options
  4. NIS-Elements HC
  5. Table of features

Never before has a software package achieved such recognition for comprehensive device control with a suite of image analysis, visualization and archiving tools.

Nikon's NIS-Elements revolutionizes imaging software for the microscopy market by combining automated intelligence to microscopes, cameras, components and peripherals with powerful archiving, analysis and visualization tools. Its intuitive interface simplifies workflow and speeds up image acquisition times while providing powerful features such as image stitching, object counting and volume views.

The NIS-Elements software suite is composed of distinct packages scaled to address specific application requirements. Each version delivers an intuitive interface to simplify workflow and provides powerful imaging tools. The software supports numerous image analysis and processing routines in conjunction with useful tools for storing and retrieving image data.

NIS-Elements AR (Advanced Research)Advanced Research

NIS-Elements AR is optimized for advanced research applications. It features fully automated acquisition and device control through full six-dimensional image acquisition and analysis.

The software is designed to deliver power and maximize workflow. It handles multi-dimensional imaging flawlessly, with support for capture, display, peripheral device control and data management and analysis of up to six dimensions (X, Y, Z, Lambda (wavelength), T, multi stage points). It also offers sophisticated image processing and visualization features, such as RAM capture, HDR image capture, intensity measurements over time, volume measurement, macro, live compare, volume view and ratio view.

The system contributes to experiment efficiency with a database building feature (option) developed to handle archiving and searching of large numbers of multi-dimensional image files.

Unified control of the entire imaging system offers significant benefits to users for cutting-edge research, such as advanced live-cell imaging.

NIS-Elements BR (Basic Research)Basic Research

NIS-Elements BR is suited for standard research applications such as analysis and photodocumentation of fluorescent imaging. It features up to four dimensional acquisition capabilities and advanced device control capabilities.

While not as full featured as the AR package, BR offers access to advanced image capture, archiving and analysis solutions that are easy to use and provide maximum workflow.

BR handles multi-dimensional imaging with ease, with support for capture, display, peripheral device control, and data management and analysis of up to four dimensions (for example: X, Y, Z, Lambda (wavelength) or X, Y, T, multipoint). It also provides advanced image processing features, such as large image (stitching), objective calibration and intensity line profile.

The system contributes to experiment efficiency with a database building feature (option) developed to handle archiving, searching, and analysis of large numbers of multi-dimensional image files. Unified control of the entire imaging system offers significant benefits to users for cutting-edge research, such as live-cell imaging.

NIS-Elements D (Documentation)Documentation

NIS-Elements D is tailored to facilitate image capture, object measurement and counting, databasing (option) and report generation. Ease of use and simplification of menu options has been emphasized in the design of the package while NIS-Elements AR and BR also display a similar look and feel.

The combination of integrated automated intelligence, streamlined workflow, attention to detail and creative design makes NIS-Elements D the perfect package for clinical and industrial applications such as tissue comparison, image archiving and reporting, particle analysis, defect analysis and fiber & textile material analysis.

Core documentation functionality includes manual length and area measurements as well as large imaging stitching. Optional modules are available: database for streamlined image filing; Extended Depth of Focus (EDF), which creates an all-in-focus image from a series of Z-axis images; live compare for comparing a live image with a static image or overlay; Automatic Measurement for automated object counting using thresholding, feature restrictions and data export; and advanced macro builder for more complex custom programming.

NIS-Elements C (Confocal Imaging)Confocal Imaging

NIS-Elements C is an optimized software package for confocal imaging. It is compatible with high-speed/high-resolution confocal microscope A1+/A1R+, multiphoton confocal microscope A1 MP+/A1R MP+, confocal microscope C2+ and spectral confocal imaging systems.

Its intuitive GUIs enable complicated sequences of experiments, such as multidimensional time-lapse imaging and photoactivation, to be carried out with simple-to-use settings. All settings and procedures required for basic confocal microscopy can be viewed in a single window.
In addition, various functions, such as filtering, averaging and image compensation, for image processing and analysis are provided.

NIS-Elements C-ER (Confocal Enhanced Resolution)Confocal Enhanced Resolution

The Enhanced Resolution (ER) system built in to the NIS-Elements C platform provides not only control of the confocal imaging and image analysis functions of the A1+/A1R+, but also provides intuitive and comprehensive image processing tools to support high resolution confocal imaging.
With the Nikon original image processing technology, high resolution confocal images can be realized with a single click operation. The software assesses the captured image and automatically determines processing parameters to achieve enhanced resolution.

NIS-Elements HC (High-Content Analysis)High-Content Analysis

NIS-Elements HC supports total operation of high-content analysis, from integrated control of motorized inverted microscope Ti-E and peripheral devices such as well plate loaders and CCD cameras, to image data management. The software interface is specifically designed for imaging multiple points within a well and across a well plate, and works with dedicated automated analysis modules.

  • Settings for well plate acquisition: several options are available for number of points, randomization and patterns, and acquisition parameters.
  • Cell count, live/dead analysis and image intensity: analysis for the purpose of generating measurement data, either during acquisition or post-acquisition.
    Cell count: automated counting of cellular objects such as nuclei or other organelles
    Live/dead: analyzes the number and percent of living and dead cells by discriminating between live and dead cell populations using two fluorescent colors, thus showing cell viability.
  • Sample labeling: links acquired images and complex experimental conditions, such as cell type labels, control wells and drug dose quantities or concentrations.
  • Plate view: a centralized view of image thumbnails. Quick access to analysis results is available with the Heat Map and Grid View functions.
  • Heat map: a customizable, graphical, color or intensity representation of analysis results well by well. A powerful tool used to visualize trends across the well plate.
  • Grid (text view): displays individual image analysis results. Filtering and exporting data to several formats such as Excel are possible.

Multi-layer Document Structure

Each document (image window) is a three-tiered layer structure, and is therefore ideally suited for analysis. Multiple-layer save is available with JPEG2000/ND2/LIM format.

NIS-Elements can work for all common file formats, such as JPEG2000, ND2, TIFF, JFF, JPG, JTF, BMP, LIM, AVI, ICS/IDS. ND2 is a special format for NIS-Elements. ND2 allows storage of sequences of images acquired during ND experiments. It contains information about the hardware settings and the experiment conditions and settings.

Supported Operation System
Windows 7 Professional SP1 (32/64-bit Version)
*NIS-Elements AR and C are only compatible with the 64-bit version.

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