Skip to sections.
New inverted microscope series at the center of bioscience’s most advanced imaging techniques. |
||||||
Scientists have overcome many live cell imaging challenges using advanced techniques such as TIRF, confocal, FRET, photo activation and microinjection. At the center of all this is the Eclipse Ti, a powerful new system that provides instant access to all these methods plus revolutionary Nikon CFI60 optics. Available in three models, the new Ti series offers improved system speeds, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging. |
||||||
| High-speed motorized control and acquisition | ||||||
The operational speeds of motorized components such as the nosepiece, fluorescence filters and stage have been greatly enhanced, allowing high-speed screening image capture during multi-dimensional experiments. Faster device movement and image acquisition reduce overall light exposure and subsequent photo-toxicity, leading to more meaningful data. The newly developed digital Controller Hub significantly increases motorized accessory speed by reducing communication overhead time between components, boosting total operation speed. |
||||||
|
||||||
| Stable time-lapse imaging with automatic focus correction system | ||||||
The Ti-E comes with a unique Perfect Focus System (PFS) that automatically corrects focus drift in real time during a prolonged period of time-lapse imaging. |
||||||
Concept of the Perfect Focus System
|
||||||
Correction to focus drift when reagents are added
|
||||||
| High-quality phase contrast images using high NA lenses | ||||||
|
||||||
| Advanced integration with peripherals via intelligent software | ||||||
Nikon's comprehensive imaging software NIS-Elements provides an integrated control of the microscope, cameras, components and peripherals. The intuitive GUI and efficient workflow make the programming of automated imaging sequences up to 6D (X, Y, Z, time, wavelength, multipoint) easy to perform. |
||||||
6D time-lapse imaging system |
||||||
| Motorized laser TIRF illumination unit | ||||||
|
||||||
Time-lapse imaging by switching TIRF and epi-fluorescence observation NG108 cell: Growth cone stained with EGFP-fascin Photos courtesy of: Satoe Ebihara, Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST) |
||||||
| Photo activation illumination unit | ||||||
|
||||||
 Photo activation of PA-GFP in a living mammalian cell by 405nm laser irradiation Photos courtesy of: Tomoki Matsuda and Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University |
||||||
| Multiport design with a maximum of five imaging ports | ||||||
Use of an optional back port enables multiple wavelength FRET imaging with multiple cameras. Moreover, by adding an eyepiece tube base unit with a side port, a maximum of five imaging ports* including left, right and bottom ports are available. (*With Ti-E/B model with bottom port) |
||||||
| Three models for a wide range of applications | ||||||
|
||||||
|
||||||
|
||||||
  |
||||||
The revolutionary external phase contrast unit incorporates a phase ring and allows the use of high NA objective lenses without a phase ring for phase contrast observation. Because there is no light loss due to a phase ring, bright "full intensity" fluorescence images as well as high-resolution phase contrast images can be captured using the same objective lens. 
The motorized laser TIRF illuminator is newly developed for Ti-E and Ti-U. The laser incident angle can be easily stored and reproduced with a single touch of the control pad button. 
The Ti-E and Ti-U feature a specialized photo activation illuminator that allows fluorescent time-lapse observation of dynamic events following photo activation or photo conversion. 
